50, 75, 100, and 150 um ID C18 Columns Global Peptide Separation Columns

High quality 50−75 um ID integrated nanoLC columns are made for mass spectrometry analysis of proteome peptides and metabolites. These columns contain uniform packing beds integrated with sharp ESI emitters to achieve high separation peak capacity (e.g., >1,000) and high sensitivity achievable for the dimension of columns.  For example, the 75 um ID columns allow confidently identifying >13,000 proteins from a 1-2 ug mammalian sample, >3,000 proteins from a 10 ng sample, and moderate to low abundance proteins from individual mammalian cells with SRM.

The high-resolution sensitive integrated columns are manufactured with use of 1.7 um and 3 um C18-bonded silica packings. The 1.7 um C18 columns fit fast peptide sequencing and targeted analysis, while 3 um long C18 columns are used to maximize analysis coverage by extension of analysis time, especially when moderate speed mass spectrometers and relatively low pressure nanoLC  instruments are applied.  Both types of columns made have a large range of column lengths, 20−80 cm for 1.7 um C18 columns and 40−120 cm for 3 um C18 columns, for achievement of the best analysis coverage in different applications.

In addition to integrated columns, we also provide separated columns for analysis of rough samples and method development.