Resource Library
CoAnn Technologies Electronic Catalog
Ultra-Sensitive NanoLC-ESI-MS for Proteomics:
“High-Efficiency Nanoscale Liquid Chromatography Coupled On-line With Mass Spectrometry Through Nanoelectrospray Ionization,” Y. Shen, R. Zhao, S.J. Berger, G.A. Anderson, N. Rodriguez, R.D. Smith, Anal. Chem. 74, 4235-4249 (2002). https://pubs.acs.org/doi/10.1021/ac0202280
“Ultra-Sensitive Nano-Scale Proteomics Using High-Efficiency On-Line MicroSPE-NanoLC-NanoESI MS and MS/MS,” Y. Shen, N. Tolic, C. Masselon, L. Paša-Tolić, D.G. Camp II, K.K. Hixson, R. Zhao, G.A. Anderson, R.D. Smith, Anal. Chem., 76, 144-154 (2004). https://pubs.acs.org/doi/10.1021/ac030096q
“Nanoscale Proteomics,” Y. Shen, N. Tolic, C. Masselon, L. Paša-Tolić, D.G. Camp II, M.S. Lipton, G.A. Anderson, R.D. Smith, Anal. Bioanal. Chem., 378, 1037-1045 (2004). https://link.springer.com/article/10.1007%2Fs00216-003-2329-8
Methods for Manipulation of Individual Cells and Small Sizes of Proteomic Samples:
“Proteomic Analysis of Single Mammalian Cells Enabled by Microfluidic Nanodroplet Sample Preparation and Ultrasensitive NanoLC-MS” Y. Zhu , G. Clair , W.B Chrisler, Y. Shen , R. Zhao , A.K Shukla , R.J. Moore, R.S Misra, G.S Pryhuber, R.D Smith, C. Ansong, R.T Kelly, Angew Chem Int Ed Engl, 57 (38), 12370-12374 (2018). https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6261339/pdf/nihms-993590.pdf
“Nanodroplet Processing Platform for Deep and Quantitative Proteome Profiling of 10–100 Mammalian Cells,” Y. Zhu, P.D. Piehowski, R. Zhao, J. Chen, Y. Shen, R.J. Moore, A.K. Shukla, V.A. Petyuk, M. Campbell-Thompson, C.E. Mathews, R.D. Smith, W-J Qian, R.T. Kelly, Nat Commun. 9, 882 (2018).
Published online 2018 Feb 28. doi: 10.1038/s41467-018-03367-w https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5830451/pdf/41467_2018_Article_3367.pdf
“Surfactant-assisted one-pot sample preparation for label-free single-cell proteomics,” C-F. Tsai, P. Zhang, D. Scholten, K. Martin, Y-T. Wang, R. Zhao, W.B. Chrisler, D.B. Patel, M. Dou, Y. Jia, C. Reduzzi, X. Liu, R.J. Moore, K.E. Burnum-Johnson, M-H Lin, C-C. Hsu, J.M. Jacobs, J. Kagan, S. Srivastava, .K.D. Rodland, H.S. Wiley, W-J Qian, R.D. Smith, Y. Zhu, M. Cristofanilli, T. Liu, H. Liu, T. Shi, Commun Biol., 4, 265 (2021). https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921383/pdf/42003_2021_Article_1797.pdf
High-Throughput Quantitative Single Cell Proteomics Protocol:
“High-Throughput Single-Cell Proteomics Analysis with Nanodroplet Sample Processing, Multiplex TMT Labeling, and Ultra-Sensitive LC-MS” K. Motamedchaboki, M. Dou, Y. Cong, R. Huguet, A.M Robitaille, Y. Shen, G. Clair, C. Ansong, D. Lopez-Ferrer, R.T. Kelly, Ying Zhu. Article on ThermoFisher website. https://assets.thermofisher.com/TFS-Assets/CMD/Application-Notes/an-65714-lc-ms-single-cell-proteomics-tmt-an65714-en.pdf
High-Resolution NanoLC MS of Proteomic Peptides:
“Automated 20 KPSI-MS and MS/MS with Chromatographic Peak Capacity of 1,000—1,500 for Proteomics and Metabolomics,” Y. Shen, R. Zhang, R.J. Moore, J. Kim, T.O. Metz, K.K. Hixson, R. Zhao, E.A. Livesay, H.R. Udseth, R.D. Smith, Anal. Chem., 77, 3090-3100 (2005). https://pubs.acs.org/doi/10.1021/ac0483062
High-Resolution NanoLC MS of Proteomic Proteins/Proteoforms:
“High-resolution ultrahigh-pressure long column reversed-phase liquid chromatography for top-down proteomics,” Y. Shen, N.Tolić, P.D.Piehowski, A.K.Shukla. S. Kim, R. Zhao, Y. Qu, E. Robinson, R.D.Smith, L. Paša-Tolić, J. Chromatogr. A. 1498, 99-110 (2017). https://www.sciencedirect.com/science/article/abs/pii/S0021967317300225
High-Resolution NanoLC MS of Polypeptides with MWs of 5000-20000 Da (Appliable for Middle-Down Proteomics):
“Strategy for degradomic-peptidomic analysis of the human blood plasma,” Y. Shen, T. Liu, N. Tolić, B.O. Petritis, R. Zhao, R.J. Moore, S.O. Purvine, D.G. Camp II, R.D. Smith, J. Proteome Res., 9, 2339-2346 (2010). https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2866148/pdf/nihms195031.pdf
“Blood peptidome-degradome profiles of breast cancer,” Y. Shen, N. Tolić, T. Liu, R. Zhao, B.O. Petritis, M.A. Gritsenko, D.G. Camp, R.J. Moore, S.O. Purvine, F.J. Esteva, R.D. Smith, PLoS ONE 5(10): e13133. doi: 10.1371/journal.pone.0013133 (2010). https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0013133&type=printable
Implementation of microSPE (trap) columns for sample loading in nanoLC MS Proteomics:
“High-Efficiency On-Line Solid-Phase Extraction Coupling to 15-150-mm-i.d. Column Liquid Chromatography for Proteomic Analysis,” Y. Shen, R.J. Moore, R. Zhao, J. Blonder, S.J. Berger, D.L. Auberry, K.J. Auberry, C. Masselon, L. Paša-Tolić, K.K. Hixson, G.A. Anderson, R.D. Smith, Anal. Chem., 75, 3596-3605 (2003). https://pubs.acs.org/doi/10.1021/ac0300690
Fast and High-Speed NanoLC MS for Ultrahigh-Throughput Proteomics:
“Ultrahigh-Throughput Proteomics Using RPLC-ESI MS/MS,” Y. Shen, R.D. Smith, K.K. Unger, D. Kumar, D. Lubda, Anal. Chem., 77, 6692-6701 (2005). https://pubs.acs.org/doi/10.1021/ac050876u
“Making Broad Proteome Protein Measurements in 1-5 min Using High-Speed RPLC Separations and High Accuracy Mass Measurements,” Y. Shen, E.F. Strittmatter, R. Zhang, T.O. Matz, R.J. Moore, F. Li, H.R. Udseth, R.D. Smith, K.K. Unger, D. Kumar, D. Lubda, Anal. Chem., 77, 7763-7773 (2005). Https://pubs.acs.org/doi/10.1021/ac051257o