15 - 75 um ID C18 Ultra-Sensitive Columns
We provide 15−75 μm ID nanoLC columns for ultrasensitive mass spectrometry proteomic analysis of a single mammalian cell (e.g., ~15 μm size, 0.1−0.2 ng protein content per cell), small populations of cells (e.g., a few to 100 mammalian cells), and tiny specimen of tissue (e.g., <0.1 mm). Combined with advanced methods of proteome sample processing and mass spectrometry analysis, these ultrasensitive nanoLC columns provide a capability to assign 1,500−5,000 proteins from 1−100 mammalian cells. These very narrow columns can also be adopted for other applications where ultrasensitive nanoLC–mass spectrometry analysis is needed.
We also provide micro-solid phase extraction columns for robust, rapid, and total recovery loading of small sizes of samples in ultrasensitive nanoLC–mass spectrometry analysis.
Note 1: Not-fitted columns (i.e., bare columns without fittings) are for users to flexibly utilize the columns according to users’ specific instrument setups, while columns with fittings (WF, 1/16” VICI-type) are for conventional nanoLC instrument configurations. 2: MicroSPE columns are typically needed for quick sample loading as these ultrasensitive columns are operated at very low flows. 3: The nanoLC operation pressure listed is for the column operated at the middle value of the flow range (at room temperature). 4: Other dimensions of USB columns can be specially ordered.